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2 edition of Effects of chloroquine on MCF7-ADR and MCF7-WT human breast cancer cell lines found in the catalog.

Effects of chloroquine on MCF7-ADR and MCF7-WT human breast cancer cell lines

Sumit Sharan

Effects of chloroquine on MCF7-ADR and MCF7-WT human breast cancer cell lines

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Published by Laurentian University in Sudbury, Ont .
Written in English


Edition Notes

Statementby Sumit Sharan.
The Physical Object
Paginationvi, 30 l. :
Number of Pages30
ID Numbers
Open LibraryOL22160664M


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Effects of chloroquine on MCF7-ADR and MCF7-WT human breast cancer cell lines by Sumit Sharan Download PDF EPUB FB2

Chloroquine sensitizes breast cancer cells to chemotherapy independent of autophagy. or in combination with other anticancer drugs. Here we show that in the mouse breast cancer cell lines, 67NR and 4T1, autophagy is induced by the DNA damaging agent cisplatin or by drugs that selectively target autophagy regulation, the PtdIns3K inhibitor Cited by:   Side effects not requiring immediate medical attention.

Some side effects of chloroquine may occur that usually do not need medical attention. These side effects may go away during treatment as your body adjusts to the medicine. Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects.

Chloroquine is a 9-aminoquinoline known since Apart from its well-known antimalarial effects, the drug has interesting biochemical properties that might be applied against some viral infections.

Chloroquine exerts direct antiviral effects, inhibiting pH-dependent steps of the replication of several viruses including members of the flaviviruses, retroviruses, and by:   Chloroquine (CQ), the worldwide used anti-malarial drug, has recently being focused as a potential anti-cancer agent as well as a chemosensitizer when used in combination with anti-cancer drugs.

It has been shown to inhibit cell growth and/or to induce cell death in various types of cancer. 5-Fluorouracil (5-FU) is the chemotherapeutic agent of first choice in colorectal cancer, but in most Cited by: The aim of the study was to examine the reversal effects of NOM on MDR in MCF7/ADR, an MCF7 breast cancer cell line resistant to adriamycin (ADR), and its mechanism of action.

The impact of anticancer activity upon Beta vulgaris extract mediated biosynthesized silver nanoparticles (ag-NPs) against human breast (MCF-7), lung (A) and pharynx (Hep-2) cancer cell lines. Venugopal K, Ahmad H, Manikandan E, Thanigai Arul K, Kavitha K, Moodley MK, Rajagopal K, Balabhaskar R, Bhaskar M.

J Photochem Photobiol B. May. WST For assessing the cytotoxic effect of α-tomatine on cell viability, WST-1 (Roche, Basel, Switzerland) reagent was used. MCF-7 cells were seeded in well microtiter plates at a concentration of 5×10 3 cells/well in μl of culture medium.

The cells were allowed to settle overnight at 37°C and in 5% COcultures were exposed to different concentrations (1, 3, 6, 9 μM) of. Enormous studies have been focused on increasing intracellular drug accumulation through inhibiting P-glycoprotein (Pgp). Meanwhile, we found that major vault protein (MVP) may be also involved in drug resistance of human breast cancer MCF-7/ADR cells by transporting doxorubicin (DOX) from the action target (i.e.

nucleus) to cytoplasma. The molecular events underlying breast cancer mechanisms of action are still poorly understood. In our study we aimed to investigate the effects of 5-FU and gemcitabine on a breast cancer cell line, MCF-7 cells, in culture on the JAK/STAT pathway using an indirect immunohistochemical method.

Materials and methods. The anticancer activity of chloroquine-gold nanoparticle conjugates (GNP-Chl) was demonstrated in MCF-7 breast cancer cells.

The MCF-7 cells were treated with different concentrations of GNP-Chl conjugates, and the cell viability was assayed using trypan blue, resulting in an IC 50 value of 30 ± 5 μg/mL. Flow cytometry analysis revealed that. An understanding of the mechanisms of apoptosis in breast cancer cells is important for clarifying of cancer pathogenesis and provides new possibilities in the treatment of breast cancer.

Therefore, we decided to study the effect of glucose shortage on apoptosis, autophagy, and their mechanisms in breast cancer MCF-7 cell line. Chloroquine side effects Get emergency medical help if you have signs of an allergic reaction (hives, difficult breathing, swelling in your face or throat) or a severe skin reaction (fever, sore throat, burning eyes, skin pain, red or purple skin rash with blistering and peeling).

This medicine may add to the chance of getting some types of cancer. Talk with the doctor. Keep away from children. Accidental exposure may cause death.

If a child takes chloroquine by accident, get medical help right away. If you are 65 or older, use chloroquine with care.

You could have more side effects. Abstract. MCF-7 human breast cancer cells provide a useful in vitro model system to study hormone-responsive breast cancer as they contain receptors for estrogen and progesterone, and estrogen both induces the synthesis of specific proteins in these cells and increases their rate of proliferation.

An MCF-7 cell line which was selected for resistance to adriamycin (MCF-7/Adr R) exhibits the. Abstract. One-third of women with breast cancer will develop bone metastases and eventually die from disease progression at these sites.

Therefore, we analyzed the ability of human MG osteoblast-like cells (MG cells), MG conditioned media (MG CM), insulin-like growth factor I (IGF-I), and transforming growth factor beta 1 (TGF-beta1) to alter the effects of adriamycin on cell cycle.

Both MCF-7/AdrR cells and the presumed parental cell line MCF-7 were included in the NCI60 panel of 60 human cancer cell lines, which has been utilized since in various pharmacological, pharmacogenomic, transcriptomic and proteomic screens.

Cell Line Origin Human Caucasian breast adenocarcinoma Cell Line Description Established from the pleural effusion from a 69 year female caucasian suffering from a breast adenocarcinoma.

Cells exhibit some features of differentiated mammary epithelium including oestradiol synthesis and formation of. Bacus SS, et al. Differentiation of cultured human breast cancer cells (AU and MCF-7) associated with loss of cell surface HER-2/neu antigen.

Mol. Carcinog. 3:PubMed: Huguet EL, et al. Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast. These side effects may go away during treatment as your body adjusts to the medicine. Also, your health care professional may be able to tell you about ways to prevent or reduce some of these side effects.

Check with your health care professional if any of the following side effects continue or are bothersome or if you have any questions about.

C2C12 cell lines expressing green fluorescent protein Other labs have also discovered this finding of RE inhibiting Akt phosphorylation in both breast cancer and liver cell lines concentration. Thus, we have uncovered multiple chloroquine effects that are separate from chloroquine’s activation of.

The doxorubicin (ADR)-selected human breast cancer cell line (MCF-7/ADR-RES) has been a useful model for a multidrug-resistant subline in cancer research.

However, a recent letter to the Journal has alerted the scientific community about the finding that MCF-7/ADR-RES is not a MCFderived cell. Stop-flow studies were used to characterize solute uptake in isolated rat lungs. These lungs were perfused at 8 or 34 ml/min for s with solutions containing I-albumin and two or more of.

The information is not intended to cover all possible uses, directions, precautions, drug interactions or adverse effects, nor should it be construed to indicate that use of a particular drug is. In human breast cancer cells, Syk stimulated intercellular aggregation, E-cadherin recruitment and retention at adherens junctions, and promoted epithelial integrity, whereas it inhibited cell migration and invasion.

Opposite effects were obtained with Syk knockdown or non-phosphorylatable mutant E-cadherin expression. Chloroquine is the drug of choice in the few malaria-endemic areas free of CRPf.

Combining chloroquine and proguanil is an option for CRPf when other first-line antimalarials are contraindicated.

Dosage should be calculated in terms of the base. The adult chloroquine dose is mg base weekly (or in some countries mg base daily).

An adriamycin-resistant human breast cancer cell line, MCF-7/ADR cells were exposed to different concentrations of PS VII (μmol/L). in a wide variety of human cancer cell lines. This paper presents the current state of knowledge on chloroquine disposition, with special emphasis on stereoselectivity and microsomal metabolism.

In addition, the impact of the patient’s physiopathological status and ethnic origin on chloroquine pharmacokinetics is discussed. In humans, chloroquine concentrations decline multiexponentially.

Chloroquine phosphate is an antimalarial and anti-inflammatory agent widely used to treat malaria and rheumatoid arthritis. Chloroquine phosphate is an autophagy and toll-like receptors (TLRs) inhibitor.

Chloroquine phosphate is highly effective in the control of SARS-CoV-2 (COVID) infection in vitro (EC50= μM). - Mechanism of Action & Protocol. The growth-inhibitory effect of cyclocreatine (CCr) and the kinetics of CCr and Na+ cotransport were investigated in MCF7 human breast cancer cells and its adriamycin-resistant subline with use of.

Chloroquine is a well-known medicine with a good safety profile that has been in use since World War 2 for the treatment of malaria and certain auto. Aralen (chloroquine) is an antimalarial drug used for the treatment of malaria and extraintestinal amebiasis. Common side effects are reduced hearing, tinnitus, nausea, vomiting, and diarrhea.

Experts theorize chloroquine could be effective against COVID coronavirus, but that is unproven. Two recent correspondences published in the Journal (1,2) labeled the MCF-7/ADR cell line—a multidrug-resistant (MDR) human breast cancer MCF-7 subline—as having a non-MCF-7 origin, which led to a change in the nomenclature of this cell line to NCI/ADR.

We believe the original nomenclature of MCF-7/ADR should be retained. Although the two MDR MCF-7 sublines (MCF. While numerous studies have evaluated the cell death inducing effect of several flavonoids in cancer cells, the effects of flavonoids on autophagy are poorly documented.

However, massive acidic vacuolization was observed upon in vitro treatment of cells with prenylflavonoids [50], [51], Quercetin [52] and Curcimin [53]. Chloroquine, a widely used antimalaria drug, is currently under investigation in clinical trials on cancer patients. It is the ability of chloroquine to inhibit autophagy in tumor cells that has.

The excretion of chloroquine and the major metabolite, desethylchloroquine, in breast milk was investigated in 11 lactating mothers following a single oral dose of chloroquine ( mg base).The maximum daily dose of the drug that the infant received from breast-feeding was about % of the maternal start dose of the drug in malaria chemotherapy.

Abstract. The effect of chloroquine (CQ) on phospholipid turnover andde novo synthesis in isolated rat mast cells (IRMC) was studied by determining the incorporation of 32 P and 14 C-glycerol into IRMC phospholipids.

Incubation of mast cells with chloroquine increased 32 P incorporation into PI and PS whilst it decreased 32 P incorporation into PC, PE and PA. In mast cells pretreated with CQ. Chloroquine impairs the replication of several viruses, including members of flaviviruses, retroviruses, and coronaviruses, by inhibiting the necessary endosome acidification upon endosomal-mediated viral entry and vesicle trafficking in the later stages of infection (i.e.

through the ER-Golgi and exocytosis from the cell. We have shown previously that ionizing radiation (IR) induces a persistent G2-M arrest but not cell death in MCF-7 breast carcinoma cells that harbor functional p53 but lack caspase In the present study, we investigated the mechanisms of apoptosis resistance and the roles of p53, caspase-3, and cell cycle arrest in IR-induced apoptosis.

The methylxanthine caffeine and the staurosporine. Studies were conducted in the sensitive and multidrug resistant human breast cancer cell lines MCF-7 and MDA/LCC6 and examined the effects of the flavonoids biochanin A, morin, phloretin, and.

Three human breast cancer cell lines, MCF7/wt, MCF7/adr (multiply drug resistant), and BT were grown to confluence, plated into 96 well dishes, and incubated with combinations of drugs for 72h.

Cell culture and treatment. The cell lines that were used in this study include MCF-7 breast cancer cells selected for resistance to doxorubicin (MCF-7 DOX) and docetaxel (MCF-7 TXT), and the non-resitant wild type parental cell line (MCF-7 CC) as we previously described (Hembruff []).Detailed selection process and characterization of these selected cell lines were described .9.

Using a 1 mL pipette, remove 1 mL of cell suspension for cell count and viability. Cell counts are performed using either an automated counter (such as Innovatis Cedex System; Beckman-Coulter ViCell system) or a hemocytometer. Record total cell count and viability.

When an automated system is. SIDE EFFECTS Special Senses Ocular. Maculopathy and macular degeneration have been reported and may be irreversible (see WARNINGS); irreversible retinal damage in patients receiving long-term or high-dosage 4-aminoquinoline therapy; visual disturbances (blurring of vision and difficulty of focusing or accommodation); nyctalopia; scotomatous vision with field defects of paracentral.